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Part:BBa_K1476003:Design

Designed by: Matthew Smith   Group: iGEM14_Reading   (2014-10-09)


PsaD deletion for Synechocystis sp. PCC 6803


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 310
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part is designed to contained regions homologous to the psaD gene. As such we had to ensure that the homologous region was of sufficient length to allow for recombination. The part contains two regions homologous to psaD: the first region is homologous to the first 250 bases of psaD, the second region to the last 250 bases of the psaD gene. The centre of the part contains a kanamycin resistance gene, which becomes inserted in the middle of the chromosomal psaD gene during recombination.


Source

Organism: Cyanobacteria Genus and Strain: Synechocystis sp. PCC 6803 Source: http://genome.microbedb.jp/cyanobase/Synechocystis/genes/slr0737 Important genotype features: none

References